Cytotoxicity of Fe 2 + , Fe 3 + , Hg 2 + , Mn 2 + , Cd 2 + , Mg 2 + , and Pb 2 + to Pc - 12 Cells
نویسندگان
چکیده
Previous studies have indicated that heavy metal exposure can have an effect on the central nervous system. However, research has been inadequate to fully support this claim. The aim of this work was to determine the toxic effects of heavy metal ions including Fe, Fe, Hg, Mn, Cd, Mg, and Pb on nerve cells (e.g. PC12). PC12 cells were incubated with the metal ions at four concentrations, i.e. 12.5 M, 25.0 M, 250 M, and 625 M. The incubation periods initially tested were 2-hour, 4-hour, and 19-hour. The 4-hour incubation period was selected for further studies. Lactate dehydrogenase (LDH) assay was used to assess the cytotoxicity of selected metals. LDH is an enzyme present in most cells that is released into the cell culture medium upon damage of the cytoplasmic membrane. PC12 cells showed signs of cytotoxicity to all metal ions tested with the exception of Mn. Mn had little to no effect on PC12 cells. Fe and Mg showed the most toxicity to PC12 cells. Interestingly, Fe was found much less toxic to PC12 cells compared with Fe. Further studies are underway to investigate their mechanism of toxicity. Future studies will include oxidative status assessment and the profiling of monoamino neurotransmitters for PC12 cells after exposure to heavy metals.
منابع مشابه
Genotoxicity, and Oxidative Stress in Pc-12 Cells
Previous studies have indicated that heavy metal exposure can have an effect on the central nervous system. However, research has been inadequate to fully support this claim. The aim of this work was to determine the toxic effects of heavy metal ions including Fe, Fe, Hg, Mn, Cd, Mg, and Pb on nerve cells (e.g. PC12). PC12 cells were incubated with the metal ions at four concentrations, i.e. 12...
متن کاملIncorporation of triazole into a quinoline-rhodamine conjugate imparts iron(III) selective complexation permitting detection at nanomolar levels.
Two new rhodamine based probes 1 and 2 for the detection of Fe(3+) were synthesized and their selectivity towards Fe(3+) ions in the presence of other competitive metal ions tested. The probe 1 formed a coloured complex with Fe(3+) as well as Cu(2+) ions and revealed the lack of adequate number of coordination sites for selective complexation with Fe(3+). Incorporation of a triazole unit to the...
متن کاملColorimetric detection of Fe3+ ions using pyrophosphate functionalized gold nanoparticles.
A sensitive, selective colorimetric Fe(3+) detection method has been developed by using pyrophosphate functionalized gold nanoparticles (P(2)O(7)(4-)-AuNPs). Gold nanoparticles were prepared by reducing HAuCl(4) with sodium borohydride, in the presence of Na(4)P(2)O(7). IR spectra suggested that pyrophosphates were capped on the surface of the gold nanoparticles. Aggregation of P(2)O(7)(4-)-AuN...
متن کاملA BODIPY-based colorimetric and fluorometric chemosensor for Hg(II) ions and its application to living cell imaging.
A new monostyryl boron dipyrromethene derivative (MS1) appended with two triazole units indicates the presence of Hg(2+) among other metal ions with high selectivity by color change and red emission. Upon Hg(2+) binding, the absorption band of MS1 is blue-shifted by 29 nm due to the inhibition of the intramolecular charge transfer from the nitrogen to the BODIPY, resulting in a color change fro...
متن کاملDual-mode unsymmetrical squaraine-based sensor for selective detection of Hg2+ in aqueous media.
A novel chemosensor based on unsymmetrical squaraine dye (USQ-1) for the selective detection of Hg(2+) in aqueous media is described. USQ-1 in combination with metal ions shows dual chromogenic and "turn-on" fluorogenic response selectivity toward Hg(2+) as compared to Li(+), Na(+), K(+), Mg(2+), Ca(2+), Ba(2+), Al(3+), Cu(2+), Cd(2+), Mn(2+), Fe(3+), Ag(+), Pb(2+), Zn(2+), Ni(2+) and Co(2+) du...
متن کامل